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Jasmonic acid and abscisic acid in shoots,coleoptiles, and roots of wheat seedlings
Authors:Wilfried Dathe  Andrew D. Parry  James K. Heald  Ian M. Scott  Otto Miersch  Roger Horgan
Affiliation:(1) Institute of Plant Biochemistry, D-06018 Halle/Saale, Germany;(2) Institute of Biological Sciences, Edward Llwyd Building, nUniversity of Wales, SY23 3DA Aberystwyth, Dyfed, UK;(3) Present address: Berlin-Chemie AG, Glienicker Weg 125-127, D-12489 Berlin, Germany;(4) Present address: Department of Biological Sciences, University of Durham, South Road, DH1 3LE Durham, UK
Abstract:The endogenous levels of abscisic acid (ABA) and jasmonic acid (JA) were analyzed in wheat seedlings grown in water, a system which in the past has been used to test the effects of these plant growth inhibitors. The levels in different plant parts and in the medium were measured by gas chromatography-mass spectrometry-selected ion monitoring, using [2H3]ABA and [2H6]JA as internal standards. In every plant part, JA levels were about 2 orders of magnitude greater than those of ABA. The exudation of JA from roots per seedling was about 14,000-fold greater than that of ABA, although the roots contained only about 170 times more JA than ABA. It is suggested that JA is a possible allelopathic compound.
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