A virus-producing cell line developed by transformation of human parapharyngeal cells with SV40 |
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Authors: | K A Rafferty Jr R L Ruben S K Young |
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Institution: | (1) Department of Anatomy, School of Basic Medical Sciences, The University of Illinois at the Medical Center, 1853 West Polk Street, 60612 Chicago, Illinois |
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Abstract: | Summary Normal cultures of epithelial appearance were initiated by trypsinization of a surgically resected, histologically normal
branchial cyst. Cellular morphology was consistent with derivation from the stratified squamous epithelium of the cyst or
from vascular endothelium, although electron micrographs of the cultured cells failed to show any junctional complexes. Infection
with SV40 produced transformants which were also epithelioid in appearance. These grew vigorously for 22 to 50 population
doublings (about 23 to 32 subcultures, depending upon regimen) and then became quiescent. During this evolution, virus was
detectable at all stages by both direct isolation (cell extracts) and cocultivation with permissive cells. In two sublines
in which selection for rapidly growing cell types occurred, virus was detected only by cocultivation. The work confirms that
of others in the finding that normal human epithelial cells are susceptible to transformation by oncogenic viruses, but are
apparently less responsive than are fibroblasts to such transforming agents. It also suggests that subcultivation techniques
that maintain the populations of transformed cells at low density tend to select against cell strains that are continuous
producers of infectious virus.
This study was supported by Grant CA 13494 from the National Cancer Institute. |
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Keywords: | human epithelial cells SV40 transformation cell culture producer lines oncogenic virus |
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