Kinetic model of lysozyme renaturation with the molecular chaperone GroEL |
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| Authors: | Xiao-Yan Dong Yu-Bing Wang Xiao-Guang Liu Yan Sun |
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| Affiliation: | (1) Department of Biochemical Engineering, Tianjin University, Tianjin, 300072, People's Republic of China;(2) The University Library, Tianjin University, Tianjin, 300072, People's Republic of China;(3) Department of Biochemical Engineering, Tianjin University, Tianjin, 300072, People's Republic of China |
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| Abstract: | From the renaturation kinetics of denatured/reduced lysozyme assisted by the molecular chaperone GroEL, a simplified kinetic model was established based on the competition between protein folding and aggregation. In the presence of GroEL and ATP, the aggregate formation was a second order reaction. With 2 mM ATP, a renaturation yield of 90% at a high renaturation rate was obtained when the molar ratio of GroEL to lysozyme was 1:1. |
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| Keywords: | GroEL kinetics lysozyme model renaturation |
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