Vital Staining with Neutral Red and Trypan Blue of 3H-Thymidine-Labeled Cells Prior to Autoradiography |
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Authors: | Wojciech Sawicki J rgen Kieler Per Briand |
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Affiliation: | a The Fibriger Laboratory, Kongens Lyngby, Denmarkb Department of Histology and Embryology, School of Medicine, Warsaw, Poland |
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Abstract: | Mouse cells cultivated in vitro were continuously labeled with tritiated thymidine for 48 hr. In representative groups of labeled cells, the percentages of dead cells were obtained by vital staining with either neutral red or trypan blue. After fixation and auto-radiography, the fraction of nonlabeled cells was determined in the same group of cells which had been used for interpretation of viability. Neither neutral red nor trypan blue used prior to autoradiography caused spurious grain formation in the emulsion. |
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