SbvI restriction endonuclease from Streptococcus bovis |
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| Authors: | I. Vanat,,P. Prista&# ,E. Kutejová ,J. Jü dová ,A. Godá ny, P. Javorský |
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| Affiliation: | Institute of Animal Physiology, Slovak Academy of Sciences, Palackého 12, Košice;*Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravskácesta 21, Bratislava, Slovak Republic |
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| Abstract: | Restriction endonuclease Sbv I, an isoschizomer of Hae III, has been isolated from rumen amylolytic bacterium Streptococcus bovis II/1. Sbv I was purified from cell extract by phosphocellulose chromatography and heparin-Sepharose chromatography. The recognition sequence of Sbv I was identified by digestion of pBR322, pUC9 and Λ-DNA and comparing the cleavage patterns obtained with computer-derived data. Sbv I recognizes the 4-bp palindrome, 5'-GGCC-3' and cleaves DNA after the second G in the sequence, producing blunt ends. |
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