Ultrasound-mediated stable transformation of potato tuber discs |
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| Authors: | Sawahel Wagdy A. |
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| Affiliation: | (1) Genetic Engineering & Biotechnology Division, National Research Centre, Cairo, Egypt;(2) Present address: International Centre for Genetic Engineering & Biotechnology, Aruna Asaf Ali Marg, 110 067 New Delhi, India |
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| Abstract: | Summary Plasmid DNA (pBARGUS), containing the selectable bar gene for resistance to the herbicide Basta, was delivered into potato tuber discs via ultrasonication. Transformed plants were identified by their ability to grow on a medium containing 1mg phosphinothricin/l. Southern hybridization and plant resistance to the application of Basta indicated that a functional bar gene had integrated into potato chromosomal DNA. |
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