Trinucleotide phosphoramidites: ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis. |
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| Authors: | B Virneks L Ge A Plückthun K C Schneider G Wellnhofer and S E Moroney |
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| Institution: | B Virnekäs, L Ge, A Plückthun, K C Schneider, G Wellnhofer, and S E Moroney |
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| Abstract: | Trinucleotide phosphoramidites representing codons for all 20 amino acids have been prepared and used in automated, solid-phase DNA synthesis. In contrast to an earlier report, we show that these substances can be used to introduce entire codons into oligonucleotides in excess of 98% yield, and are ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis. |
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