A comparative assessment of purification techniques for mesophyll protoplasts of Brassica napus L. |
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Authors: | Stephen Millam Alan T. H. Burns Trevor J. Hocking |
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Affiliation: | (1) Cell and Molecular Genetics Department, Scottish Crop Research Institute, Invergowrie, DD2 5DA Dundee, Scotland;(2) School of Applied Science, The Polytechnic, WV1 1SB Wolverhampton, England |
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Abstract: | The effects of three different general purification protocols have been assessed quantitatively using mesophyll protoplasts of Brassica napus. Within the initial sample two distinct sub-populations were determined. The methods used influenced the ratio of the vacuolated to chloroplastic type protoplast sub-populations. Overall recovery rates of the initial sample varied according to the method used from 38% to 27%, but the relative recovery of the sub-populations varied considerably with a purified ratio of between 1.0:0.78 to 1.0:7.0. Size distribution profiles of the initial and purified populations are also presented. |
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Keywords: | Brassica napus protoplasts purification |
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