Two threonine residues required for role of AfsKav in controlling morphogenesis and avermectin production in Streptomyces avermitilis |
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Authors: | Rajkarnikar Arishma Kwon Hyung-Jin Ryu Yeon-Woo Suh Joo-Won |
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Institution: | Department of Biological Science, Institute of Bioscience and Biotechnology, Myongji University, Yongin 449-728, Korea. |
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Abstract: | AfsKav is a eukaryotic-type serine/threonine protein kinase, required for sporulation and avermectin production in Streptomyces avermitilis. In terms of their ability to complement SJW4001 (DeltaafsK-av), afsK-av mutants T165A and T168A were not functional, whereas mutants T165D and T168D retained their ability, indicating that Thr-165 and Thr-168 are the phosphorylation sites required for the role of AfsKav. Expression of the S-adenosylmethione synthetase gene promoted avermectin production in the wild-type S. avermitilis, yet not in the mutant harboring T168D or T165D, demonstrating that tandem phosphorylation on Thr-165 and Thr-168 in AfsKav is the mechanism modulating avermectin production in response to S-adenosylmethione accumulation in S. avermitilis. |
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