Monoclonal antibody directed against RNA polymerase II of Drosophila melanogaster |
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| Authors: | Angela Krä mer, Regina Haars, Rainer Kabisch, Hans Will, Friedlinde A. Bautz Ekkehard K. F. Bautz |
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| Affiliation: | (1) Molekulare Genetik, Universität Heidelberg, Im Neuenheimer Feld 230, D-6900 Heidelberg, Federal Republic of Germany;(2) Present address: Department of Biological Chemistry, Washington University, School of Medicine, St. Louis, 63 110, Missouri, USA;(3) Present address: Institut für Strahlenchemie, Max-Planck-Institut für Kohleforschung, D-4330 Mülheim, Federal Republic of Germany |
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| Abstract: | Summary Monoclonal antibodies were raised against purified RNA polymerase II (or B) from Drosophila melanogaster. The antibody produced by one hybridoma cell clone was found to be directed against the two large subunits of the enzyme. The absence of antibodies directed against proteins possibly contaminating the antigens used for immunization allowed us to identify RNA polymerase unequivocally in interbands and puffs of polytene chromosomes. Within a single heat shock puff (87C1) RNA polymerase was found to be clustered in two separate areas suggesting two distint regions of RNA polymerase activity in this puff.Abbreviations FITC fluorescein isothiocyanate - PAGE polyacrylamide gel electrophoresis - PBS phosphate buffered saline - SDS sodium dodecyl sulfate - Enzyme DNA-dependent RNA polymerase or nucleotide-triphosphate - RNA nucleotidyltransferase (EC 2.7.7.6) |
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