In vitro sensitization to transplantation antigens. V. Suppression of T-cell and of B-cell function in vitro |
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| Authors: | J W Dyminski B F Argyris |
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| Affiliation: | 1. Clinical Neuroscience Lab, Institute of Psychology, Polish Academy of Sciences, Warsaw, Poland;2. Department of Psychology, Institute of Psychiatry, Psychology and Neuroscience, King''s College London, United Kingdom;1. Institute of Physiology and Pathophysiology, University of Heidelberg, Heidelberg, Germany;2. Department of Neuroradiology, Heidelberg University Hospital, Heidelberg, Germany;1. Center for Music in the Brain, Department of Clinical Medicine, Aarhus University, Denmark;2. Center for Eudaimonia and Human Flourishing, University of Oxford, UK;3. Computational Neuroscience Group, Pompeu Fabra University, Spain |
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| Abstract: | A previous report from this laboratory indicated that thymus-bone marrow cooperation occurs during the in vitro sensitization to transplantation antigens. The present report continues our investigation of this T and B cell interaction by attempting to control each of these cell populations in vitro.Two lines of evidence indicate that the T cell is required during the in vitro sensitization to alloantigens: (1) Anti-theta serum pretreatment of lymphoid cells interferes with their ability to become sensitized in vitro to transplantation antigens; (2) Lymphoid cells from neonatally thymectomized donors do not become allosensitized in vitro.In vitro suppression of B cell function was accomplished with an antibone marrow serum (anti-beta serum). Anti-beta serum treatment of lymph node or bone marrow cells impairs the ability of such cells to partcipate in the in vitro sensitization to transplantation antigens.The active cell subpopulations of lymph node cells, obtained by density gradient fractionation, can likewise be suppressed in vitro by pretreatment with anti-theta or anti-beta serum.These results confirm the necessity for thymus-marrow cooperation in vitro, and in addition suggest the existence, in the lymph node, of subclasses of T and B cells participating in the in vitro allograft reaction. |
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