Action of insulin analogues on cultured human fibroblasts reflects biological potency. |
| |
Authors: | M D Hollenberg |
| |
Affiliation: | Division of Clinical Pharmacology, Departments of Pharmacology and Experimental Therapeutics, and Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA |
| |
Abstract: | The biological sensitivity of cultured human skin-derived fibroblasts was examined in terms of the ability of insulin and insulin analogues to stimulate the uptake of alpha-aminoisobutyrate in these cells. The relative biological activity measured: insulin=desalanine insulin > proinsulin > desoctapeptide insulin parallels exactly the relative activity of these compounds on fat and muscle preparations both and . Inactive insulin analogues do not stimulate alpha-aminoisobutyrate uptake. It is concluded that the chemical specificity of human fibroblast insulin receptors is retained in culture and that the biological responsiveness (alpha-aminoisobutyrate uptake) of such cells to insulin should prove useful for comparative studies of receptors obtained from different individuals. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|