Association of the kinesin motor KIF1A with the multimodular protein liprin-alpha |
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Authors: | Shin Hyewon Wyszynski Michael Huh Kyung-Hye Valtschanoff Juli G Lee Jae-Ran Ko Jaewon Streuli Michel Weinberg Richard J Sheng Morgan Kim Eunjoon |
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Affiliation: | Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea. |
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Abstract: | Liprin-alpha/SYD-2 is a multimodular scaffolding protein important for presynaptic differentiation and postsynaptic targeting of alpha-amino-3-hydroxy-5-methyl-4-isoxazoleproprionic acid glutamate receptors. However, the molecular mechanisms underlying these functions remain largely unknown. Here we report that liprin-alpha interacts with the neuron-specific kinesin motor KIF1A. KIF1A colocalizes with liprin-alpha in various subcellular regions of neurons. KIF1A coaccumulates with liprin-alpha in ligated sciatic nerves. KIF1A cofractionates and coimmunopreciptates with liprin-alpha and various liprin-alpha-associated membrane, signaling, and scaffolding proteins including alpha-amino-3-hydroxy-5-methyl-4-isoxazoleproprionic acid receptors, GRIP/ABP, RIM, GIT1, and beta PIX. These results suggest that liprin-alpha functions as a KIF1A receptor, linking KIF1A to various liprin-alpha-associated proteins for their transport in neurons. |
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