体外肿瘤微血管生成模型的建立

本研究以体外微血管培养模型为基础，用鼠尾胶原包埋大鼠动脉环，并将包埋的动脉环转移到种有人肺癌A549细胞单层的培养皿中，用MCDB 131无血清培养液对动脉环和肿瘤细胞进行共培养，从而建立肿瘤微血管体外生成模型。大鼠动脉环于培养后第3天从血管壁长出微血管芽，第6至10天长成微血管丛，两周后新生微血管开始萎缩；没有肿瘤细胞刺激的条件下，大鼠动脉环新生微血管数量明显减少。结果表明人肺癌A549细胞能够促进血管生成。体外大鼠动脉环肿瘤微血管培养模型操作简单、灵敏度高，适合研究肿瘤血管新生及其机制。

Setting up of the tumor angiogenesis model in vitro

Tumor angiogenesis model in vitro was set up by embedding the rat aortic rings in collegen and culturing them in serum-free MCDB 131 culture medium with or without lung cancer A549 cells. This model was based on the rat aortic ring model. In both control cultures and cultures supplemented with lung cancer A549 cells, most of the microvessels sprouted from the cut edges of the aortic rings. Microvessels usually appeared by the third day, at their maximal growth by days 6~10 and began to degenerate by the end of the second week, but cultures supplemented with lung cancer A549 cells could generate more microvessels than control. It concluded from current study that Lung cancer A549 cells could promote angiogenesis. The rat aortic ring model is simple and sensitive. It is a promising meathod to study angiogenesis and its mechanisms.