氧化LDL的不同组分在抑制巨噬细胞释放NO中的作用

研究表明：氧化ＬＤＬ（Ｏｘ－ＬＤＬ）可抑制ＬＰＳ诱导的巨噬细胞ＮＯ释放，而正常ＬＤＬ和乙酰化ＬＤＬ则无抑制作用。用谷胱甘肽地的酶模拟物ｅｂｓｅｌｅｎ清除Ｏｘ－ＬＤＬ上的脂氢过氧化物对其抑制作用没有影响。Ｏｘ－ＬＤＬ的蛋白组分对ＮＯ释放也没有影响，而脂质组分则有抑制作用。ＬＤＬ脂质的主要成分亚油酸和卵磷脂无论单独还是共同氧化后对ＮＯ释放都没有影响，而亚油酸和胆固醇一起氧化后则对ＮＯ的释放有很强的抑制

Sequencing of Lipoprotein Lipase Gene in 11 Cases of Chinese Patients With Severe Endogenous Hypertriglyceridemia

Sequencing analysis of lipoprotein lipase ( LPL ) gene exons 1 9 was made in 11 cases of Chinese patients with severe endogenous hypertriglyceridemia (serum TG >7.68 mmol/L) by the dideoxy chain termination method using Sequenase PCR Product Sequencing Kit. Four of the patients were found to possess 3 variants at the LPL gene locus.One novel mutation was observed at exon 8 Thr 361 Thr (C 1338 A). This was a conservative mutation with an allele frequency of 11.4% in the 74 of the Chinese controls.Two others Ala 261 Thr (G 1037 A) in exon 6 and Ser 447 Ter (C 1595 G) in exon 9, which were also found in Caucasians and have been reported to have disease associations with dyslipidaemia,showed at frequencies of 0% and 10.7% in the Chinese controls respectively.However, it is the first time that these three genetic variants have been reported in Chinese subjects.The results show that the gene variation of LPL may not be associated with endogenous hypertriglyceridemia in Chinese population.