Analysis of growth and production coupling for batch cultures of Lactobacillus helveticus with the help of an unstructured model |
| |
| Institution: | 1. Military Nutrition Division, US Army Research Institute of Environmental Medicine, Natick, MA, USA;2. Oak Ridge Institute for Science and Education, Belcamp, MD, USA;3. MyoSyntax Corporation, Worcester, MA, USA;4. University of California at Berkeley, Berkeley, CA, USA;5. Defence Research and Development Canada, University of Toronto, Canada;6. Pennington Biomedical Research Center, Baton Rouge, LA, USA;7. Duke University, Durham, NC, USA;1. Institute of Animal Nutrition, Department of Veterinary Medicine, Freie Universität Berlin, Germany;2. Current address: Small Animal Clinic, Faculty of Veterinary Medicine, Justus-Liebig-University Giessen, Germany |
| |
| Abstract: | An unstructured model has been developed by assuming a sigmoidal variation with time of specific growth rate and introducing an additional term in Luedeking and Piret production kinetics. The model fitted growth and production kinetic data. The model was helpful for examining the influence of initial yeast extract concentration on growth and production coupling. In richly supplemented media, the growth associated production mechanism prevailed, while in the case of poor nitrogen supplementation, most lactic acid production was non-growth-associated. Both contributions might be calculated from model parameters; this was especially interesting when the forms of growth and production kinetics did not allow a clear conclusion. The additional term in the production rate expression was a convenient way to characterize ‘usable nitrogen’ concentration in the medium. From the model a clear criterion has been derived for optimizing the nitrogen supplementation from an economic point of view. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
