Impact of process parameters on chitinase production by an alkalophilic marine Beauveria bassiana in solid state fermentation |
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| Institution: | 2. Department of Physics, Chemistry and Mathematics, Federal University of São Carlos, Sorocaba, SP, Brazil;3. Department of Chemistry, Federal University of São Carlos, São Carlos, SP, Brazil;4. Department of Genetics and Evolution, Federal University of São Carlos, São Carlos, SP, Brazil;1. Persian Gulf and Oman Sea Ecology Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research Education and Extension Organization (AREEO), Bandar Abass, Iran;2. Department of Marine Biology, Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, Iran |
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| Abstract: | A chitinolytic fungus, Beauveria bassiana was isolated from marine sediment and significant process parameters influencing chitinase production in solid state fermentation using wheat bran were optimised. The organism was strongly alkalophilic and produced maximum chitinase at pH 9·20. The NaCl and colloidal chitin requirements varied with the type of moistening medium used. Vegetative (mycelial) inoculum was more suitable than conidial inoculum for obtaining maximal enzyme yield. The addition of phosphate and yeast extract resulted in enhancement of chitinase yield. After optimisation, the maximum enzyme yield was 246·6 units g?1 initial dry substrate (U gIDS?1). This is the first report of the production of chitinase from a marine fungus. |
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