Recovery of pectolytic enzymes produced by solid state culture of Aspergillus niger |
| |
| Affiliation: | 1. Program of Chemical Engineering, COPPE, Federal University of Rio de Janeiro, Ilha do Fundão, Centro de Tecnologia, sala G-116, 21949-900, Rio de Janeiro, Brazil;2. Department of Chemical Engineering, School of Chemistry, Federal University of Rio de Janeiro, Ilha do Fundão, Centro de Tecnologia, sala E-209, 21949-900, Rio de Janeiro, Brazil;1. Laboratory of Cell and Molecular Tumor Biology and Bioactive Compounds, São Francisco University Medical School (USF), Bragança Paulista, São Paulo, Brazil;2. Department of Pathology, University of Campinas (Unicamp), Campinas, São Paulo, Brazil;3. Ghent University Hospital, Department of Neurosurgery, Ghent, Belgium;4. ABC Medical School, Santo André, São Paulo, Brazil;5. Post-Graduate Program, Department of Neurosurgery, State Civil Servant Hospital (IAMSPE), São Paulo, São Paulo, Brazil;1. Department of Otolaryngology–Head and Neck Surgery, University of Michigan, 1540 E Hospital Dr., Ann Arbor, MI, 48109, USA;2. Department of Biomedical Engineering, University of Michigan, 2200 Bonisteel Blvd., Ann Arbor, MI, 48109, USA;3. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, 313 Ferst Drive, Atlanta, GA, 30332, USA;1. Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Colima, Col 28045, Mexico;2. CONACYT, Facultad de Medicina, Universidad Autónoma de San Luis Potosí, San Luis Potosí, SLP 78210, Mexico;3. Department of Physiology and Biophysics, Virginia Commonwealth University, School of Medicine, Richmond, VA 23298, USA;4. Departamento de Fisiología y Biofísica, Facultad de Medicina, Universidad Autónoma de San Luis Potosí, SLP 78210, Mexico |
| |
| Abstract: | Pectinases are enzymes with a wide range of applications in the food and drink industries. In the present work, the extraction of pectinases produced by Aspergillus niger in a solid state fermentation system was investigated. The purpose was to reduce enzyme losses in the fermented solids and at the same time obtain a crude extract as concentrated as possible. Initially the performances of stirred tank and fixed bed extractors were compared. Polygalacturonase activity and viscosity reducing capacity obtained in the stirred tank system were 105% and 15% superior, respectively. Repeated extractions and multiple stage countercurrent extraction were studied, employing stirred tanks. It was possible to observe that three stages were enough for total recovery of the enzymes contained in the solids. The final enzyme extract obtained by counter-current extraction with three stages showed a polygalacturonase activity 81% higher than the one obtained by one-stage extraction. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
