Production of biomass and lutein by Chlorella protothecoides at various glucose concentrations in heterotrophic cultures |
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| Affiliation: | 1. College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, China;2. College of Oceanography and Food Science, Quanzhou Normal University, Quanzhou 362000, China;3. Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan;4. Research Center for Energy Technology and Strategy, National Cheng Kung University, Tainan 701, Taiwan;5. College of Mechanical Engineering and Automation, Fuzhou University, Fuzhou 350108, China;1. University Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan;2. Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan;3. Department of Biotechnology, National Kaohsiung Normal University, Kaohsiung, Taiwan;4. Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan;5. Research Center for Energy Technology and Strategy Center, National Cheng Kung University, Tainan, Taiwan;1. College of Agriculture, Forestry and Natural Resource Management, University of Hawaii at Hilo, 200 W. Kawili Street, Hilo, HI, 96720, USA;2. Department of Construction Environmental Research, Korea Institute of Construction Technology, 2311 Daehwa-Dong, Ilsan-gu, Kyonggi-do, Republic of Korea;3. Department of Civil Engineering, Inha University, 100 Inha-ro, Nam-gu, Incheon, Republic of Korea;1. Department of Food Engineering, Federal University of Paraíba, João Pessoa, Paraíba 58051-900, Brazil;2. Department of Veterinary Sciences, University of Pisa, Viale delle Piagge, 2, Pisa 56124, Italy;3. Interdepartmental Research Center “Nutraceuticals and Food for Health”, via del Borghetto, 80, Pisa 56124, Italy;4. Department of Biology, University of Pisa, via Luca Ghini, 5, Pisa 56126, Italy |
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| Abstract: | The influence of initial glucose concentrations on the production of biomass and lutein by Chlorella protothecoides CS-41 was investigated in batch cultures using both shake flasks and fermentors. The maximum biomass concentration increased from 4·9 to 31·2 g litre−1 dry cells with an increase in initial glucose concentration from 10 to 80 g litre−1. An even higher initial glucose concentration (100 g litre−1) resulted in a lower biomass concentration, a lower specific growth rate, a lower growth yield coefficient and a considerably longer lag phase, which might be due to substrate inhibition. The initial glucose level also had a significant effect on the production of lutein. In a 3·7-litre fermentor an increase in lutein production from 19·39 to 76·56 mg litre−1 was obtained with an increase in initial glucose concentration from 10 to 40 g litre−1, within which range, lutein yield coefficient was constant (YItn=1·90±0·02 mg g−1). A simple substrate inhibition model was developed, which fitted the experimental data better than the classical Haldane model. A group of time-dependent kinetic models for algal cultivation in fermentors were also constructed, which were in good agreement with the experimental results and could be employed to predict the production of biomass and lutein, and the consumption of glucose in fermentor cultures. |
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