Production of bacterial exopolysaccharides by solid substrate fermentation |
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Affiliation: | 1. Instituto de Bioquímica y Medicina Molecular (IBIMOL)-Universidad de Buenos Aires (UBA), CONICET, Fisicoquímica, Facultad de Farmacia y Bioquímica, Junín 956 (C1113AAD), Buenos Aires, Argentina;2. Instituto Antártico Argentino, 25 de Mayo 1143, San Martin (CP1650), Buenos Aires, Argentina;3. Universidad Nacional de Luján, R5 y Av. Constitución (CP6700), Luján, Buenos Aires, Argentina;4. Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, Am Handelshafen 12, D-27570 Bremerhaven, Germany;5. Museo Argentino de Ciencias Naturales “B. Rivadavia”, Av. A. Gallardo 470 (C1405DJR), Buenos Aires, Argentina;2. Laboratory for Molecular Microbiology, Department of Marine Biotechnology, Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Ensenada, Baja California, Mexico |
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Abstract: | In a comparison of submerged cultivation (SC) with solid substrate fermentation (SSF) for the production of bacterial exopolysaccharides (EPS), the latter technique yielded 2 to 4.7 times more polymer than the former, on the laboratory scale. SSF was performed using inert solid particles (spent malt grains) impregnated with a liquid medium. The polymer yields obtained from SSFs, as referred to the impregnating liquid volumes, were as follows: 38.8 g/litre xanthan from Xanthomonas campestris, 21.8 g/litre succinoglycan from Rhizobium hedysari and 20.3 g/litre succinoglycan from Agrobacterium tumefaciens PT45. These results make this technique promising for a potential application on the industrial scale. A further advantage with this fermentation process is found in the availability and low cost of substrates, which are obtained as by-products or wastes from the agriculture or food industry. |
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