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Ethanol production in solid substrate fermentation using thermotolerant yeast
Affiliation:1. Department of Microbiology, Osmania University, Hyderabad 500 007, A.P, India;2. Regional Research Laboratory, CSIR, Trivandrum 695019, Kerala, India;1. Department of Environmental Engineering, Zhejiang Ocean University, No. 1 Haida South Road, Zhoushan 316022, China;2. Department of Environmental Engineering, Zhejiang University, Hangzhou, 310058, China;3. School of the Environment, Nanjing University, Nanjing 210023, China;1. Department of Regional Environmental Research Center, National Institute for Environmental Studies, 16−2 Onogawa, Tsukuba, Ibaraki 305−8506, Japan;2. Department of Civil and Environmental Engineering, Nagaoka University of Technology, 1603−1 Kamitomioka, Nagaoka, Niigata 940−2188, Japan;3. Department of Social and Environmental Engineering, Hiroshima University, 1−4−1 Kagamiyama Higashi, Hiroshima 739−8527, Japan;4. Department of Civil and Environmental Engineering, National Institute of Technology, Kisarazu College, 2−11−1 Kiyomidaihigashi, Kisarazu, Chiba 292−0041, Japan;5. New Industry Creation Hatchery Center, Tohoku University, 6−6−04 Aoba, Aramakiaza, Aoba-ku, Sendai, Miyagi 980−8579, Japan;1. Key Laboratory of Yangtze River Water Environment, Ministry of Education, Tongji University, Siping Road, Shanghai 200092, PR China;2. State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science & Engineering, Tongji University, Shanghai 200092, PR China;1. Nelson Mandela African Institution of Science and Technology, P.O. Box 447, Arusha, Tanzania;2. One Health Centre, School of Veterinary Medicine, University of California, Davis, CA, USA;1. Department of Civil and Environmental Engineering, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata 940-2188, Japan;2. New Industry Creation Hatchery Center, Tohoku University, 6-6-04 Aza-Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan;3. Department of Science of Technology Innovation, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata 940-2188, Japan;1. Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, China;2. Zhejiang Province Key Laboratory for Water Pollution Control and Environmental Safety, Hangzhou 310058, China;3. Department of Environmental Engineering, Zhejiang Ocean University, No. 1 Haida South Road, Zhoushan 316022, China;4. Haining Second Waterworks Co. Ltd., Haining 314408, China
Abstract:A novel solid substrate fermentation system was used to produce fuel ethanol from sweet sorghum and sweet potato using a thermotolerant Saccharomyces cerevisiae strain (VS3) and a local isolate of amylolytic Bacilllus sps. (VB9). The process was carried out on a laboratory scale using broth cultures. Alcohol produced was estimated by gas chromatography after an incubation time of 72 h at 37 and 42°C. More ethanol was produced in co-culture with a mixed substrate than with the thermotolerant yeast (VS3) alone. The maximum amount of ethanol produced in co-culture with a mixed substrate was 5 g/100 g of substrate at 37°C and 3·5 g/100 g of substrate at 42°C.
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