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Utilization of the tricarboxylic acid cycle intermediates and symbiotic effectiveness inRhizobium meliloti
Authors:H Antoun  L M Bordeleau  Renee Sauvageau
Institution:(1) Département des sols, Faculté des Sciences de l'Agriculture et de l'Alimentation, Université Laval, G1K 7P4 Québec, Canada;(2) Station de recherches, Agriculture Canada, G1V 2J3 Sainte-Foy, Québec, Canada
Abstract:Summary The utilization of the tricarboxylic acid cycle intermediates and related compounds was studied in strains ofRhizobium meliloti having different symbiotic effectiveness. In general, the very effective (VE) strains used these compounds as sole carbon source better than the ineffective (I) strains. However, a significant different was observed between VE and I strains in their ability to use acetate or oxaloacetate for growth. In fact, at a concentration of 2 mM, 80% of the VE strains used acetate or oxaloacetate white 50% of the I strains used acetate and none was able to grow on oxaloacetate. No correlation was found between the symbiotic effectiveness of the strains and their ATP content, when grown on mannitol. The highest ATP content (9.21 nM×mgrg protein–1) was found in the I strain S20 and the lowest (0.69 nM×mgrg protein–1 was found in the effective strain S8. Numerical analysis of the patterns of utilization of the TCA cycle intermediates and related compounds indicated that the 49 strains tested formed 11 distinct groups at 86% similarity, according to Jaccard's coefficient. Several strains showed unique patterns of utilization and can be clearly identified under laboratory conditions.Contribution no.225 Station de Recherches, Agriculture Canada.
Keywords:ATP  Bacteria  Lucerne  Medicago sativa  Plant  Rhizobium meliloti  Strain identification  Symbiotic effectiveness  TCA intermediates utilization
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