Effects of Insert Size on Transposition Efficiency of the Sleeping Beauty Transposon in Mouse Cells |
| |
| Authors: | Attila Karsi Boaz Moav Perry Hackett Zhanjiang Liu |
| |
| Institution: | (1) Fish Molecular Genetics and Biotechnology Laboratory, Department of Fisheries and Allied Aquacultures and Program of Cell and Molecular Biosciences, Auburn University, Auburn, AL 36849, U.S.A., US;(2) Department of Zoology, Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel, IL;(3) Department of Genetics, Cell Biology, and Development, University of Minnesota, St. Paul, MN 55108, U.S.A., US |
| |
| Abstract: | Transposon vectors are widely used in prokaryotic and lower eukaryotic systems. However, they were not available for use
in vertebrate animals until the recent reconstitution of a synthetic fish transposon, Sleeping Beauty (SB). The reacquisition of transposability of the SB transposase fostered great enthusiasm for using transposon vectors as tools in vertebrate animals, particularly for gene
transfer to facilitate accelerated integration of transgenes into chromosomes. Here, we report the effects of insert sizes
on transposition efficiency of SB. A significant effect of insert size on efficiency of transposition by SB was found. The SB transposase enhanced the integration efficiency effectively for SB transposon up to approximately 5.6 kb, but lost its ability to enhance the integration efficiency when the transposon size
was increased to 9.1 kb. This result indicates that the SB transposon system is highly applicable for transferring small genes, but may not be applicable for transferring very large
genes.
Received October 20, 2000; accepted December 15, 2000. |
| |
| Keywords: | :Tcl-like integration gene transfer transgenic fish |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
