Expression and purification of recombinant vesicular glutamate transporter VGLUT1 using PC12 cells and High Five insect cells |
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Authors: | Email author" target="_blank">S?ren?S?L?AndersenEmail author |
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Institution: | (1) Department of Neurology and Neurological Sciences, Stanford University, 94305-5489 Stanford, CA, USA |
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Abstract: | In synaptic vesicles, the estimated concentration of the excitatory amino acid glutamate is 100–150 mM. It was recently discovered
that VGLUT1, previously characterized as an inorganic phosphate transporter (BNPI) with 9–11 predicted transmembrane spanning
domains, is capable of transporting glutamate. The expression and His-tag based purification of recombinant VGLUT1 from PC12
cells and High Five insect cells is described. Significantly better virus and protein expression was obtained using High Five
rather than Sf9 insect cells. PC12 cell expressed VGLUT1 is functional but not the Baculovirus expressed protein. The lack
of functionality of the Baculovirus expressed VGLUT1 is discussed. The data indicate that VGLUT1 readily oligomerizes/dimerizes.
The data are discussed in the context of developing this system further in order to reconstitute vesicular glutamate uptake
in vitro using lipid-detergent vesicles.
Published: June 7, 2004. |
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Keywords: | Indexing terms" target="_blank">Indexing terms Membrane Proteins Baculovirus Glutamates |
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