| Affiliation: | (1) Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstrasse 3, 06466 Gatersleben, Germany;(2) Institut für Mikrobiologie, Heinrich-Heine-Universität Düsseldorf, Universitätsstrasse 1, 40225 Düsseldorf, Germany;(3) Umweltforschungszentrum Leipzig-Halle (UFZ), Permoserstrasse 15, 04318 Leipzig, Germany;(4) Rhein Biotech, Eichsfelder Strasse 11, 40595 Düsseldorf, Germany;(5) Present address: Med Artis Pharmacenticals, Pauwelstraße 19, 52047 Aachen, Germany |
| Abstract: | An Arxula adeninivoransintegration vector was applied to a range of alternative yeast species including Saccharomyces cerevisiae, Debaryomyces hansenii, Debaryomyces polymorphus, Hansenula polymorpha and Pichia pastoris. The vector harbours a conserved A. adeninivorans-derived 25S rDNA sequence for targeting, the A. adeninivorans-derived TEF1promoter for expression control of the reporter sequence, and the Escherichia coli-derived hph gene conferring resistance against hygromycin B for selection of recombinants. Heterologous gene expression was assessed using a green fluorescent protein (GFP) reporter gene. The plasmid was found to be integrated into the genome of the various hosts tested; recombinant strains of all species exhibited heterologous gene expressions of a similar high level. |
