Improved purification procedure and some serological and physica properties of cassava common mosaic virus from South America |
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Authors: | BARRY L. NOLT ANA CECILIA VELASCO BENJAMIN PINEDA |
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Affiliation: | Centra International de Agricultura Tropical, Apartado Aereo 67 13, Cali, Colombia |
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Abstract: | Large quantities of cassava common mosaic virus (CCMV) were purified from systemically infected Nicotiana benthamiana plants. A polyclonal antiserum, with a titre of 1/128 in the tube precipitin test, was produced by immunising rabbits with purified virus. Viral antigens were detected in cassava, using both the double-antibody sandwich or plate-trapped antigen forms of enzyme-linked immunosorbent assay (ELISA). The virus reacted with antisera to the potexviruses potato virus X and tulip virus X in F(ab')2 ELISA. As determined by ELISA, isolates of CCMV from cassava and chaya are closely serologically related to each other. Leaf extracts from infected N. benthamiana plants were infective to a dilution of 10--4 but not 10--5; after heating for 10 min at 65 °C but not 70 °C; and after storage at room temperature for 14 days. The virus has a sedimentation coefficient of 126 S20,w, a single coat protein molecule of c . mol. wt 21 000, and a single-stranded RNA genome of c . mol. wt 2.0 ± 106. Several dsRNA species, including the putative viral replicative form of c . mol. wt 4.1 ± 106, were isolated from virus-infected cassava and N. benthamiana . |
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Keywords: | Cassava common mosaic virus purification properties detection |
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