Cloning of the Lentinula edodes B mating-type locus and identification of the genetic structure controlling B mating |
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Authors: | Lin Wu Arend van Peer Wenhua Song Hong Wang Mingjie Chen Qi Tan Chunyan Song Meiyan Zhang Dapeng Bao |
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Affiliation: | 1. Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, P.R. China;2. Iwate Biotechnology Research Center, Iwate, Japan;3. Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, P.R. China;4. National Engineering Research Center of Edible Fungi, Ministry of Science and Technology, P.R. China |
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Abstract: | During the life cycle of heterothallic tetrapolar Agaricomycetes such as Lentinula edodes (Berk.) Pegler, the mating type system, composed of unlinked A and B loci, plays a vital role in controlling sexual development and resulting formation of the fruit body. L. edodes is produced worldwide for consumption and medicinal purposes, and understanding its sexual development is therefore of great importance. A considerable amount of mating type factors has been indicated over the past decades but few genes have actually been identified, and no complete genetic structures of L. edodes B mating-type loci are available. In this study, we cloned the matB regions from two mating compatible L. edodes strains, 939P26 and 939P42. Four pheromone receptors were identified on each new matB region, together with three and four pheromone precursor genes in the respective strains. Gene polymorphism, phylogenetic analysis and distribution of pheromone receptors and pheromone precursors clearly indicate a bipartite matB locus, each sublocus containing a pheromone receptor and one or two pheromone precursors. Detailed sequence comparisons of genetic structures between the matB regions of strains 939P42, 939P26 and a previously reported strain SUP2 further supported this model and allowed identification of the B mating type subloci borders. Mating studies confirmed the control of B mating by the identified pheromone receptors and pheromones in L. edodes. |
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Keywords: | AA, amino acid(s) AF, aliphatic amino acids (or alanine)&ndash phenylalanine bp, base pair(s) CAAX, cysteine&ndash aliphatic amino acids&ndash aliphatic amino acids&ndash any residue Cc, Coprinopsis cinerea CIIA, cysteine&ndash isoleucine&ndash isoleucine&ndash alanine CIIS, cysteine&ndash isoleucine&ndash isoleucine&ndash serine CVIL, cysteine&ndash valine&ndash isoleucine&ndash leucine CVIS, cysteine&ndash valine&ndash isoleucine&ndash serine CVVA, cysteine&ndash valine&ndash valine&ndash alanine Cn, Cryptococcus neoformans CTAB, Cetyltrimethyl Ammonium Bromide DDE, aspartic acid&ndash aspartic acid&ndash glutamic acid dNTP, deoxyribonucleoside triphosphate E/A, glutamic acid/alanine E/H, glutamic acid/histidine ER, glutamic acid&ndash arginine Fv, Flammulina velutipes GF, glycine&ndash phenylalanine GY, glycine&ndash tyrosine GX, glycine&ndash any residue kb, kilobase(s) or 1000 bp Le, Lentinula edodes Lb, Laccaria bicolor NCBI, National Center for Biotechnology Information No, number PCR, polymerase chain reaction PDA, Potato, Dextrose and Agar medium PDY, Potato, Dextrose and Yeast extract medium PXD, proline&ndash any residue&ndash aspartic acid PXN, proline&ndash any residue&ndash aspartic acid&ndash asparaginate TM, transmembrane XF, any residue&ndash phenylalanine |
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