Genetic variants in BMP8B gene are associated with growth traits in Chinese native cattle |
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Authors: | Xiu-Kai Cao Jing Wang Xian-Yong Lan Chu-Zhao Lei Chun-Lei Zhang Xing-Lei Qi Hong Chen |
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Institution: | 1. College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China;2. Institute of Cellular and Molecular Biology, Jiangsu Normal University, Xuzhou, Jiangsu 221116, China;3. Bureau of Animal Husbandry of Biyang County, Biyang, Henan 463700, China |
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Abstract: | As a signaling molecule, bone morphogenetic protein 8B (BMP8B) plays an essential role in bone metabolism and is able to regulate thermogenesis and energy balance, which suggests that BMP8B gene may be a new candidate for growth traits. Here, to characterize the effects of BMP8B gene on growth traits, we first used three Chinese indigenous cattle breeds (n = 845) to detect single nucleotide polymorphisms (SNPs). Five novel SNPs of BMP8B gene (g.− 242C>T, g.2164C>T, g.2639T>C, g.2900C>G and g.10817C>T) were identified by DNA pool sequencing and forced PCR–RFLP. And then we associated the five SNPs with four growth traits (body weight, body length, heart girth, and hucklebone width). Results from association analysis showed that the SNPs 1, 2, and 3 affected growth trait(s) markedly (P < 0.05). Further, 6 combined haplotypes were constructed to guarantee the reliability of analysis results. There were also significant differences in body length, heart girth and body weight between the 6 combined haplotypes (P < 0.05), but not in hucklebone width (P > 0.05). Collectively, our results suggest a modulatory role of BMP8B gene in cattle growth and development, and 3 SNPs could be used as molecular markers in early marker assisted selection (MAS) in beef cattle breeding program. |
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Keywords: | Asp aspartic acid BAT brown adipose tissue bp base pair(s) BMPs bone morphogenetic proteins GLM General Linear Model Hap haplotype He observed heterozygosity Ho observed homozygosity HWE Hardy&ndash Weinberg equilibrium JX Jiaxian cattle LD linkage disequilibrium LE linkage equilibrium MAS marker assisted selection Ne effective allele numbers NY Nanyang cattle PCR&ndash RFLP polymerase chain reaction&ndash restricted fragment length polymorphisms PGC primordial germ cell PIC polymorphism information content QC Qinchuan cattle SE standard error SNP(s) single nucleotide polymorphism(s) TBE a buffer solution containing a mixture of Tris base boric acid and EDTA TGF-β transforming growth factor-beta |
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