ChAy/Bx, a novel chimeric high-molecular-weight glutenin subunit gene apparently created by homoeologous recombination in Triticum turgidum ssp. dicoccoides |
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Authors: | Xiao-Hui Guo Zhe-Guang BiBi-Hua Wu Zhen-Zhen WangJi-Liang Hu You-Liang ZhengDeng-Cai Liu |
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Affiliation: | Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, China; Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Ya''an 625014, China |
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Abstract: | High-molecular-weight glutenin subunits (HMW-GSs) are of considerable interest, because they play a crucial role in determining dough viscoelastic properties and end-use quality of wheat flour. In this paper, ChAy/Bx, a novel chimeric HMW-GS gene from Triticum turgidum ssp. dicoccoides (AABB, 2n = 4x = 28) accession D129, was isolated and characterized. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis revealed that the electrophoretic mobility of the glutenin subunit encoded by ChAy/Bx was slightly faster than that of 1Dy12. The complete ORF of ChAy/Bx contained 1671 bp encoding a deduced polypeptide of 555 amino acid residues (or 534 amino acid residues for the mature protein), making it the smallest HMW-GS gene known from Triticum species. Sequence analysis showed that ChAy/Bx was neither a conventional x-type nor a conventional y-type subunit gene, but a novel chimeric gene. Its first 1305 nt sequence was highly homologous with the corresponding sequence of 1Ay type genes, while its final 366 nt sequence was highly homologous with the corresponding sequence of 1Bx type genes. The mature ChAy/Bx protein consisted of the N-terminus of 1Ay type subunit (the first 414 amino acid residues) and the C-terminus of 1Bx type subunit (the final 120 amino acid residues). Secondary structure prediction showed that ChAy/Bx contained some domains of 1Ay subunit and some domains of 1Bx subunit. The special structure of this HMW glutenin chimera ChAy/Bx subunit might have unique effects on the end-use quality of wheat flour. Here we propose that homoeologous recombination might be a novel pathway for allelic variation or molecular evolution of HMW-GSs. |
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Keywords: | aa, amino acid(s) bp, base pair(s) CTAB, cetyltrimethyl ammonium bromide dNTPs, deoxyribonucleoside triphosphates HMW-GS, high-molecular-weight glutenin subunit LMW-GS, low-molecular-weight glutenin subunit InDels, insertions and deletions IPTG, isopropyl-β-Δ-thiogalactopyranoside ORF, open reading frame PCR, polymerase chain reaction SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis SNP, single nucleotide polymorphism GISH, genomic in situ hybridization NCBI, National Center for Biotechnology Information |
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