Potential dependence of the admittance ofChara plasmalemma |
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Authors: | M J Beilby B N Beilby |
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Institution: | (1) Botany School, University of Cambridge, CB2 3EA Cambridge, England |
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Abstract: | Summary A computer-controlled apparatus is described, which combines the two powerful methods of voltage-clamping and admittance measurement.
The 5-Hz admittance ofChara plasmalemma is obtained for transmembrane PD from −400 mV to 0. DC conductance is also measured by the bipolar staircase
method. Both the DC and 5-Hz conductances at steady state display a central maximum at ≈−250 mV. This feature is attributed
to the conductance/voltage characteristics of the H+ pump. The steady-state capacitance does not show any trend throughout the potential interval.
At the time of the delay, before excitation commences, the 5-Hz conductance is smaller than after excitation.
At the time of excitation the 5-Hz conductance echoes the time-course of the ionic current, while the capacitance undergoes
a sharp decrease followed by an increase. A possible explanation of the capacitance behavior is attempted involving transport
number effects and reactances associated with the Hodgkin-Huxley gating mechanism.
At punchthrough the membrane becomes inductive. |
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Keywords: | Chara voltage clamp admittance proton pump action potential punchthrough transport number effects |
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