Metabolism of leukotriene B4 in hepatic microsomes |
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Authors: | J F Newton R Eckardt P E Bender T Leonard K Straub |
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Affiliation: | 1. Department of Laboratory Medicine & Pathology, Mayo Clinic, Rochester, MN, United States;2. Department of Medicine, Division of Allergic Diseases, Mayo Clinic, Rochester, MN, United States;3. Mayo Clinic Program for Mast Cell and Eosinophil Disorders, Mayo Clinic, Rochester, MN, United States |
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Abstract: | Leukotriene B4 was metabolized in rat hepatic microsomes to two products. Mass spectral analysis of these two metabolites indicated that the major metabolite was the 20-hydroxy metabolite while the minor metabolite was the 19-hydroxy metabolite. The formation of these metabolites required NADPH and was linear with time (20 min) and protein (1.6 mg/ml). The Km apparent and Vmax for omega hydroxylation of LTB4 was 14 uM and 0.138 nmol/min/mg protein. In contrast, the km and Vmax for omega minus one hydroxylation was 54 uM and 0.093 nmol/min/mg protein. These results suggest that omega and omega minus one hydroxylations of LTB4 may be mediated by different isozymes of hepatic P-450. |
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