Diversity of "Candidatus Liberibacter asiaticus," based on the omp gene sequence |
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Authors: | Bastianel C Garnier-Semancik M Renaudin J Bové J M Eveillard S |
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Institution: | Laboratoire de Biologie Cellulaire et Moléculaire, UMR GDPP, INRA, Université Victor Segalen Bordeaux 2, 71 avenue Edouard Bourlaux BP81, 33883 Villenave D'Ornon Cedex, France. |
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Abstract: | Huanglongbing (yellow dragon disease) is a destructive disease of citrus. The etiological agent is a noncultured, phloem-restricted alpha-proteobacterium, "Candidatus Liberibacter africanus" in Africa and "Candidatus Liberibacter asiaticus" in Asia. In this study, we used an omp-based PCR-restriction fragment length polymorphism (RFLP) approach to analyze the genetic variability of "Ca. Liberibacter asiaticus" isolates. By using five different enzymes, each the 10 isolates tested could be associated with a specific combination of restriction profiles. The results indicate that the species "Ca. Liberibacter asiaticus," even within a given region, may comprise several different variants. Thus, omp-based PCR-RFLP analysis is a simple method for detecting and differentiating "Ca. Liberibacter asiaticus" isolates. |
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