Conversion of the lipoprotein secreted by cultured eel hepatocytes to high density lipoprotein |
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| Institution: | 1. Pearl River Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, China Ministry of Agriculture, Guangzhou 510380, China;2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;3. Guangdong Liangshi Aquatic Seed Industry Co., Ltd, Foshan 528100, China;1. Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China;2. Guangdong Science and Innovation Center for Pearl Culture, Zhanjiang 524088, China;3. Pearl Breeding and Processing Engineering Technology Research Centre of Guangdong Province, Zhanjiang 524088, China;4. Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang 524088, China;5. Guangdong Marine Ecology Early Warning and Monitoring Laboratory, Zhanjiang 524088, China;1. Laboratory of Aquatic Animal Nutrition and Feed, Fisheries College, Guangdong Ocean University, Zhanjiang, China;2. Guangdong Wei Lai Biotechnology Co., Ltd, Guangzhou 510000, China;3. Guangxi Academy of Marine Sciences, Guangxi Academy of Sciences, Nanning, China;4. Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, China;5. Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang, China |
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| Abstract: | - 1.1. The lipoprotein, a VLDL-like lipoprotein, secreted by cultured eel hepatocytes was incubated with whole eel serum, serum HDL, or serum VLDL. No change in the VLDL-like lipoprotein was found.
- 2.2. The secreted lipoprotein was incubated with five kinds of liposomes and a HDL-like particle was formed in the presence of BSA only when l-α-dimyristoyl lecithin liposome was used.
- 3.3. In the presence of 3% BSA, apo AI, proapo AI, apo AII and apo C of the secreted lipoprotein were transferred to the l-α-dimyristoyl lecithin liposome and a HDL-like particle was formed.
- 4.4. The secreted lipoprotein was hydrolyzed by lipoprotein lipase and a HDL-like particle formed after hydrolysis contained no triglyceride and had phospholipid as its main lipid.
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