Purification and properties of betaine aldehyde dehydrogenase from Xanthomonas translucens |
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| Institution: | 1. College of Materials Science & Engineering Zhejiang University of Technology, Hangzhou 310014, PR China;2. College of Biological, Chemical Science and Technology Jiaxing University, Jiaxing 314001, PR China;1. Catalysts and Organic Synthesis Research Laboratory, Department of Chemistry, Iran University of Science and Technology, Tehran, 16846-13114, Iran;2. Mechanical Engineering Department, Sahand University of Technology, Tabriz, Iran;3. Protein Chemistry Laboratory, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran;4. School of Mechanical Engineering, Iran University of Science and Technology (IUST), Tehran, Iran;5. Organic Chemistry Laboratory, Department of Chemistry, Tarbiat Modares University, Tehran, 14155-4838, Iran;6. Pharmaceutical and Heterocyclic Compounds Research Laboratory, Department of Chemistry, Iran University of Science and Technology, Tehran, 16846-13114, Iran;7. School of Chemistry, College of Science, University of Tehran, Tehran, Iran;8. Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA, 02114, USA;9. Department of Dermatology, Harvard Medical School, Boston, MA, 02115, USA;10. Laser Research Centre, Faculty of Health Science, University of Johannesburg, Doornfontein, 2028, South Africa;11. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran;12. Department of Biotechnology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran;13. Drug Design and Bioinformatics Unit, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran;1. School of Bio-Chemical Engineering and Technology, Sirindhorn International Institute of Technology, Thammasat University, Pathum Thani, Thailand;2. Program in Chemical Biology, Chulabhorn Graduate Institute and the Center of Excellence on Environmental Health, Toxicology and Management of Chemicals, 54 Kamphaeng Phet 6, Talat Bang Khen, Lak Si, Bangkok 10210, Thailand;3. Laboratory of Medicinal Chemistry, Chulabhorn Research Institute (CRI), 54 Kamphaeng Phet 6, Talat Bang Khen, Lak Si, Bangkok 10210, Thailand;1. Dipartimento di Biochimica, Biofisica e Patologia Generale, Università degli Studi della Campania “Luigi Vanvitelli”, Via L. De Crecchio 7, 80138 Napoli, Italy;2. Stazione Sperimentale per le Industrie delle Essenze e dei derivati dagli Agrumi, Azienda Speciale della Camera di Commercio di Reggio Calabria, Via Generale Tommasini 2, 89127 Reggio Calabria, Italy;3. Ministero dello Sviluppo Economico, Via Molise 2, Roma, Italy;4. Dipartimento di Ingegneria Industriale e ProdALscarl, Università degli Studi di Salerno, Via Ponte Don Melillo 1, 84084 Fisciano, Salerno, Italy;5. Dipartimento di Medicina Veterinaria e Produzioni Animali, Università degli Studi di Napoli Federico II, Via Delpino 1, 80137 Napoli, Italy;2. Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, Australia |
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| Abstract: | Betaine aldehyde dehydrogenase from Xanthomonas translucens was purified to apparent homogeneity by ammonium sulfate fractionation, followed by ion-exchange, butyl-Toyopearl and gel filtration chromatography. The amino acid composition and the N-terminal sequence of 35 amino acid residues were determined. The enzyme was found to be a tetramer with identical 50 kDa subunits. Both NAD and NADP could be used as a cofactor for the enzyme and Km values for NAD and NADP were 70 μM and 50 μM, respectively. The enzyme was highly specific for betaine aldehyde and the Km value for betaine aldehyde was 0.19 mM. |
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