In vivo quantification of cholesterol content in human carotid arteries by quantitative Gamma-camera imaging after injection of autologous low density lipoproteins (LDL) |
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| Affiliation: | 1. Université Claude Bernard Lyon 1, UMR CNRS 5246, ICBMS, Univ Lyon, LYON, France;2. CNRS, INSERM, l''institut du thorax, Nantes Université, Nantes, France;3. Department of Internal Medicine I, Cardiology, Medical Faculty, RWTH Aachen University, Aachen Germany;4. Laboratoire des IMRCP, Université de Toulouse, CNRS UMR 5623, Université Toulouse III, Paul Sabatier, France;5. INSERM, Unit 1033, LYOS, Lyon, France;6. CERMEP-Imaging Platform, Bron, France;7. Sanford Burnham Prebys, La Jolla, California;8. Stroke Department, Hospices Civils de Lyon, France;9. CarMeN Laboratory, INSERM, INRA, INSA Lyon, Université Claude Bernard Lyon 1, Univ Lyon, Lyon, France;10. Laboratory of Cancer Biology and Molecular Immunology, Faculty of Sciences-I, Lebanese University, Beirut, Lebanon;11. PRASE and Biology Department, Faculty of Sciences - I, Lebanese University, Beirut, Lebanon;12. Brain and Cognition Research Center CerCo, CNRS UMR5549, Université de Toulouse, France;1. State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, Macao 999078, China;2. Department of Pharmaceutical Sciences, Faculty of Health Sciences, University of Macau, Taipa, Macao 999078, China;3. National Integrated Traditional and Western Medicine Center for Cardiovascular Disease, China-Japan Friendship Hospital, Beijing 100029, China |
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| Abstract: | Low density lipoprotiens (LDL) were isolated by immunoaffinity chromatography from 18 patients (31–70 years) suffering from primary hypercholesterolemia with angiographically proven atherosclerosis of either one or both carotid arteries. LDL were labeled with 123I (1 mCi/mg LDL) by the iodine monochloride method followed by purification with dialysis and immediately reinjected thereafer. Gamma-camera serial controls over carotid regions allowed visual detection of uptake of the radiocompound uptake in 12 out of the 18 patients. The lipid entry ratio (LER; counts over the vascular region/pixel as compared to the contralateral side after background subtraction) confirmed the visual findings. Whole body images performed until 20 h after reinjection showed 3 different kinetic types of LDL-influx into the vessel wall: decreasing (type I), increasing and then decreasing (type II) and continuously increasing (type III) with time. Four patients underwent endarterectomy within 2–7 weeks after gamma-camera imaging. Histological control revealed an extensive amount of “foam cells” in tissue samples derived during surgery and an absence of endothelial lining in samples belonging to patients with type II kinetics. |
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