Limitations on cell volume determination using three-dimensional optical microscopy |
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| Affiliation: | 1. ‘Dongzao’ Research Institute of Zhanhua District, Binzhou Shandong 256800, China;2. Research Center of Chinese Jujube, Hebei Agricultural University, Baoding Hebei 071001, China;3. College of Horticulture, Hebei Agricultural University, Baoding Hebei 071001, China;4. School of Landscape and Ecological Engineering, Hebei University of Engineering, Handan Hebei 056038, China;1. Flanders Marine Institute (VLIZ), InnovOcean Site, Wandelaarkaai 7, 8400 Ostend, Belgium;2. Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Animal Sciences Unit - Aquatic Environment and Quality, Ankerstraat 1, 8400 Ostend, Belgium;3. Department of Animal Sciences and Aquatic Ecology, Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Coupure Links 653, 9000 Gent, Belgium;4. Department of Animal Sciences and Aquatic Ecology, GhEnToxLab, Ghent University, Coupure Links 653, 9000 Gent, Belgium;1. Department of Genetics, Faculty of Biology, Sofia University “St. Kliment Ohridski”, 1164 Sofia, 8 Dragan Tsankov Blvd., Sofia, Bulgaria;2. MAICh, Department of Hοrticultural Genetics and Biοtechnology, Makedοnias 1, Chania 73100, Greece |
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| Abstract: | One important application for three-dimensional microscopy is the determination of the volumes of biological cells. In this paper we investigate the application of three-dimensional deconvolution techniques to the problem of cell volume determination. The theoretical discussion is based on a Fourier sampling condition for reliable image reconstruction and it is shown, using this condition and simulated images, that the cell volume determination is reliable only for cells with a shape and orientation such that their length along the optic axis is much greater than their width. |
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