Isolation of crustacean egg yolk lipoproteins by differential density gradient ultracentrifugation |
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| Affiliation: | 1. Sorbonne Universités, UPMC Univ Paris 06, Inserm, Université Paris Descartes, Sorbonne Paris Cité, UMR_S 1138, Centre de recherche des Cordeliers, F-75006 Paris, France;2. EPHE, Ecole Pratique des Hautes Etudes, Laboratoire de Pharmacologie Cellulaire et Moléculaire, F-75014 Paris, France;1. School of Biotechnology, Dublin City University, Republic of Ireland;2. Department of Molecular Systems Biology, UFZ - Helmholtz Centre for Environmental Research, Leipzig, Germany;3. Department of Effect-Directed Analysis, UFZ - Helmholtz Centre for Environmental Research, Leipzig, Germany;4. Department of Bioanalytical Ecotoxicology, UFZ - Helmholtz Centre for Environmental Research, Leipzig, Germany;5. School of Nursing, Psychotherapy, and Community Health, Dublin City University, Republic of Ireland;1. Department of Hydrobiology, Faculty of Biology, University of Warsaw at Biological and Chemical Research Centre, Żwirki i Wigury 101, 02-089, Warsaw, Poland;2. Department of Biochemistry and Microbiology, Institute of Biology, Warsaw University of Life Sciences (SGGW), Nowoursynowska 159, 02-776, Warsaw, Poland;3. Department of Animal Physiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland |
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| Abstract: | - 1.1. Egg yolk lipoproteins from four species of Crustacea were isolated by differential density gradient ultracentrifugation.
- 2.2. Egg yolk proteins from freshwater prawn, striped stone crab and mitten crab consissted of high-density lipoprotein (HDL) and lipid-free protein, while low-density lipoprotein (LDL) was present in the egg yolk protein of sand crayfish as well as HDL and lipid-free protein.
- 3.3. HDL was a major component in the egg yolk proteins from four species of Crustacea. HDL was identical to egg yolk lipovitellin.
- 4.4. Both HDL and LDL possessed phospholipid as a major lipid.
- 5.5. HDL, but not LDL, contained carotenoids. The color of HDL from mitten crab showed a reddish purple and was distinct from other Crustacea whose color was orange. The reddish purple color was characterized by an absorption flexion at 600–650 nm.
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