Genome complexity of the maize rust fungus,Puccinia sorghi |
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| Institution: | 1. Division of Plant Industry, CSIRO, GPO Box 1600, Canberra, ACT 2601, Australia;2. Department of Plant Pathology, University of California, Davis, California 95616 USA;1. Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), Faculty of Natural and Agricultural Sciences, University of Pretoria, Hatfield Campus, Pretoria 0083, South Africa;2. Biotechnology Platform, Agricultural Research Council (ARC), Onderstepoort Campus, Pretoria 0110, South Africa;1. Applied Physics Department, The Hebrew University of Jerusalem, Jerusalem 91904, Israel;2. The Harvey M. Krueger Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel;1. School of Plant Sciences Haramaya University, P.O. Box 138, Dire Dawa, Ethiopia;2. CIMMIYT –Ethiopia, ILRI Campus, P.O. Box 5689, Addis Ababa, Ethiopia;3. Kawazulu Natal University, P.O. Box 3470, South Africa;4. Norwegian Institute of Bioeconomy Research, P.O. Box 115, Oslo, Norway;1. Department of Biochemistry, Genetics and Microbiology (BGM), Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa;2. School of Life Sciences, University of Nevada, Las Vegas, Nevada, United States;3. National Institute for Communicable Disease, National Health Laboratory Service, Johannesburg, South Africa;4. Department of Biochemistry and Microbiology, Institute of Biology, Warsaw University of Life Sciences (SGGW), Poland;1. Department of Plant and Soil Sciences, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, South Africa;2. Department of Biochemistry, Genetics and Microbiology, FABI, University of Pretoria, South Africa;3. Department of Applied Science, Maseno University, Kenya;1. Division of Plant and Soil Sciences, West Virginia University, Morgantown, WV, 26506, USA;2. School of Forest Resources and Conservation, University of Florida, Gainesville, FL, 32611, USA;3. Division of Forestry and Natural Resources, West Virginia University, Morgantown, WV, 26506, USA |
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| Abstract: | The base composition and complexity of genomic DNA fromPuccinia sorghi have been estimated by thermal denaturation, analytical ultracentrifugation, and reassociation kinetics. The buoyant density of genomic DNA in CsCl was found to be 1.7021 g/ml, which corresponds to a GC content of 43%. From thermal denaturation curves the GC content was estimated to be 41%. The haploid genome size ofP. sorghi was estimated to be 4.7 × 107 bp, half of which represented a moderately repetitive fraction. The size of theP. sorghi genome is similar to that of other basidiomycete fungi; however, the amount of repetitive DNA is greater than that reported for most other fungi. |
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