An improved method of direct labeling monoclonal antibodies with 99mTc |
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| Institution: | 1. Dartmouth College, Tuck School of Business, Hanover, NH 03755, USA;2. Norwegian School of Economics, Helleveien 30, Bergen 5045, Norway;3. European Corporate Governance Institute, c/o the Royal Academies of Belgium, Palace of the Academies, Brussels 1000, Belgium;4. US Securities and Exchange Commission, 100 F Street, NE, Washington, DC 20549, USA;5. Centre for Economic Policy Research, 33 Great Sutton Street, London EC1V 0DX, UK;1. Materials Science Division, Lawrence Livermore National Laboratory, Livermore, CA, United States;2. Computational Engineering Division, Lawrence Livermore National Laboratory, Livermore, CA, United States;1. S.C.I.C.P.E. BISTRITA S.A., Bistrita, Romania;2. University of Bucharest, Research Institute of the University of Bucharest-ICUB, Bucharest, Romania;1. Department of Neuroscience (DNS), University of Padova, Padua, Italy;2. Padua Neuroscience Center, University of Padova, Padua, Italy;3. Department of Medicine-DIMED, Radiology Institute, University of Padova, Azienda Ospedale-Università Padova, Padua, Italy;4. Department of Molecular Medicine, University of Padova, Padua, Italy;5. Department of Neurosciences, Otolaryngology Section University of Padova, Padua, Italy;6. Neuroradiology Unit, Neurosciences Department, University of Padova, Azienda Ospedale-Università Padova, Padua, Italy;7. Parkinson and Movement Disorders Unit, Study Center for Neurodegeneration (CESNE), Department of Neurosciences, University of Padova, Padua, Italy |
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| Abstract: | An improved method of direct labeling MAbs with 99mTc is described. Two murine monoclonal antibodies, designated Lym-1 and B72.3, have been successfully labeled with 99mTc in 0.1 M borate buffer at pH 9.3. The choice of buffer and pH was essential for obtaining a radiolabeling yield ⩾98%. In vitro studies demonstrated that the radiolabeled antibodies were stable and retained their immunoreactivity. Imaging and biodistribution studies using Raji and LS174T human tumor-bearing nude mice demonstrated a significant tumor uptake at 24-h post-injection of 99mTc-labeled MAbs. This improved labeling method showed better stability than those of previously published methods and resulted in significant improvement in the uptake of antibody in tumor. External images at 24 h post-injection revealed clearly visible tumors demonstrating the benefit of this method for tumor immunoscintigraphy. |
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