Structures of ribosomal subunits from Saccharomyces cerevisiae |
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| Institution: | 1. Programa de Pós-Graduação em Bioexperimentação, Universidade de Passo Fundo, BR 285, São José, Passo Fundo, Rio Grande do Sul, Brazil;2. Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil;1. Department of Pharmacy, School of Pharmaceutical Sciences, University of São Paulo, Brasil, Avenida Prof. Lineu Prestes, 580, Cidade, Universitária;2. Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, Brasil, Avenida Prof. Lineu Prestes, 580, Cidade, Universitária;1. School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus, PMB1, Glen Osmond, South Australia 5064, Australia;2. CSIRO Land and Water, PMB 2, Glen Osmond, SA 5064, Australia;3. Ventia Pty Ltd, North Sydney, New South Wales 2060, Australia;4. Queensland Alliance for Environmental Health Sciences (QAEHS), The University of Queensland, Brisbane, Queensland 4102, Australia;5. Department of Civil and Environmental Engineering, Colorado School of Mines, Golden, CO 80401, USA |
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| Abstract: | The large (60S) and small (40S) ribosomal subunits were isolated from yeast (Saccharomyces cerevisiae), and preparations stained with uranyl acetate were imaged by transmission electron microscopy. Averages of three different projections of each subunit were obtained by computerized image processing with reproducible spatial resolutions approaching 3.0 and 3.5 nm, respectively. Similarities between the structures of these particles and those of other eukaryotic ribosomal subunits contribute to the development of a concensus model for the eukaryotic ribosome. |
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