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Absorption and blood clearance of labelled carotenoids ([14C]astaxanthin, [3H]Canthaxanthin and [3H]zeaxanthin) in mature female rainbow trout (Oncorhynchus mykiss)
Institution:1. Institut für Populationsgenetik, Vetmeduni Vienna, Veterinärplatz 1, Wien, A-1210, Austria;2. Vienna Graduate School of Population Genetics, Wien, A-1210, Austria;3. Institut für Tierzucht und Genetik, Vetmeduni Vienna, Veterinärplatz 1, Wien, A-1210, Austria;4. Centre of Biological Diversity, School of Biology, University of St. Andrews, St Andrews KY16 9TH, UK
Abstract:
  • 1.1. Using one force-fed meal, eight mature female rainbow trout received 14C]astaxanthin (14C]Ax) with 3H]canthaxanthin (3H]Cx; N = 3) or with 3H]zeaxanthin (3H]Zx; N = 5).
  • 2.2. Approximately 200 μl of blood were collected via caudal puncture every 24 hr for 4 days. After 96 hr, the fish were killed and pyloric caeca (P.C.) from the duodenal intestine (D.I.) section, ileal intestine (I.I.), and posterior intestine (P.I.) were dissected out.
  • 3.3. In the blood, Ax levels were higher than Cx followed by Zx levels.
  • 4.4. This corresponds to their respective absorption by the trout as was confirmed by their relative concentrations in P.C., I.I. and P.I.
  • 5.5. However, blood clearance was similar for all three compounds. 14C]Phoenicoxanthin (14C]Px) was detected as a reduced metabolite of 14C]Ax in all gut sections.
Keywords:
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