Production and partial properties of an extracellular polysaccharide from Streptomyces sp. A-1845 |
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| Institution: | 1. Bio-Med Big Data Center, CAS Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China;2. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai 200241, China;3. Shanghai Laboratory Animal Research Center, Shanghai 201203, China;4. Institute for Digital Health, International Human Phenome Institutes (Shanghai), Shanghai 200433, China;5. Shanghai Center for Bioinformation Technology, Shanghai Academy of Science and Technology, Shanghai 201203, China;6. School of Computer Science and Technology, Harbin Institute of Technology (Shenzhen), Shenzhen, Guangdong 518055, China;7. Shanghai Institute of Immunology, Department of Immunology and Microbiology, Shanghai JiaoTong University School of Medicine, Shanghai 200025, China;8. State Key Laboratory of Genetic Engineering, Ministry of Education Key Laboratory of Contemporary Anthropology, Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Fudan University, Shanghai 200438, China;9. Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, Zhejiang 330106, China;1. Institute of Atmospheric Sciences and Climate, National Research Council, ISAC-CNR, 73100 Lecce, Italy;2. Department of Environmental and Biological Sciences and Technologies (DISTEBA), University of Salento, 73100 Lecce, Italy;1. Department of Mechanical Engineering, National Institute of Technology Calicut, Kerala, 673601, India;2. Department of Chemical Engineering, National Institute of Technology Calicut, Kerala, 673601, India;1. College of Materials Science and Engineering, Jilin Institute of Chemical Technology, Jilin City 132022, Jilin Province, PR China;2. College of Materials and Textile Engineering & Nanotechnology Research Institute (NRI), Jiaxing University, Jiaxing City 314001, Zhejiang Province, PR China;3. Department of Radiology, Samsung Medical Center, Sungkyunkwan University, School of Medicine and Center for Molecular and Cellular Imaging, Samsung Biomedical Research Institute, Seoul 06351, the Republic of Korea;1. Guangxi Key Laboratory of Electrochemical and Magneto-chemical Functional Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541006, China;2. State Key Laboratory Base of Eco-Chemical Engineering College of Chemical Engineering, Qingdao University of Science & Technology, Qingdao 266042, China;3. Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education), Nankai University, Tianjin 300071, China |
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| Abstract: | An extracellular polysaccharide was isolated from culture broth of Streptomyces sp. A-1845 by ethanol precipitation, DEAE-Toyopearl column chromatography and gel filtrations in Toyopearl HW75f and HW65s. The purified polysaccharide gave a single peak on Toyopearl HW65s gel filtration. Nitrogen and phosphorus content of the purified preparation were 4 and 2.5%, respectively. It was composed of d-mannose, d-galactose, d-galacturonic acid, d-xylose, d-glucosamine, l-rhamnoe, d-glucose, l-fucose, d-ribose and d-galactosamine in the ratio of 7.6, 4.0, 3.4, 3.1, 2.6, 1.9, 1.7, 1.1, 1.0 and 0.6. |
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