The antifertility agent,gossypol, changes several mitochondrial functions in the presence of Mg2+ |
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| Institution: | 1. State Key Laboratory of Medicinal Chemical Biology and Frontiers Science Center for Cell Responses, College of Life Sciences, Nankai University, Tianjin 300071, China;2. Institute for Immunology, College of Life Sciences, Nankai University, Tianjin 300071, China;3. State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Nankai University, Tianjin 300350, China;4. Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China;5. School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia;6. Innovative Center for Pathogen Research, Guangzhou Laboratory, Guangzhou 510005, China;7. National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences (CAS), Beijing 100101, China;8. Laboratory of Structural Biology, Tsinghua University, Beijing 100084, China |
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| Abstract: | 1. The effect of gossypol in the presence of K+ or Mg2+, or both, was studied on ATPase activity and respiration of rat liver mitochondria.2. Respiration was uncoupled in the presence of gossypol, Mg2+, and K+, whereas in the presence of gossypol and Mg2+ a partial inhibition was observed.3. Gossypol stimulated ATPase activity in the presence of K+ or Mg2+, but maximal activity was observed when both cations were in the incubation medium.4. Stimulation of ATPase activity in the presence of Mg2+ was dose related.5. EDTA reverted the stimulation produced by gossypol on ATPase activity.6. Gossypol had no effect on the ATPase activity of submitochondrial particles, which suggests an indirect action of gossypol on the enzyme.7. Mitochondrial membrane potential showed a higher collapse in the presence of gossypol and 1mM MgCl2.8. The observed effects of gossypol could be explained by the collapse of the mitochondrial membrane potential. |
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