Cumene hydroperoxide-induced chemiluminescence in human erythrocytes: Effect of antioxidants and sulfhydryl compounds |
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| Affiliation: | 1. Laboratory for Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, the Netherlands;2. RNA Biology & Applied Bioinformatics, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, the Netherlands;1. Amrita Center for Nanoscience and Molecular Medicine, Amrita Institute of Medical Sciences and Research Center, Amrita Vishwa Vidyapeetham, AIMS – Ponekkara, Cochin 682041, Kerala, India;2. Department of Microbiology, Amrita Institute of Medical Sciences and Research Center, Amrita Vishwa Vidyapeetham, AIMS – Ponekkara, Cochin 682041, Kerala, India;1. Center of Reproductive Medicine, Shengjing Hospital of China Medical University, Shenyang, 110004, China;2. Department of Plastic Surgery, The First Affiliated Hospital of China Medical University, Shenyang, China;3. Key Laboratory of Reproductive and Genetic Medicine (China Medical University), National Health Commission, Shenyang, 110004, China;4. Key Laboratory of Reproductive Dysfunction Diseases and Fertility Remodeling of Liaoning Province, Shenyang, 110004, China;1. State Key Laboratory of Pollution Control and Resource Reuse, School of Environment, Nanjing University, Nanjing 210023, PR China;2. Department of Environmental Sciences, The Connecticut Agricultural Experiment Station, New Haven, CT 06511, USA;3. Research Center for Environmental Nanotechnology (ReCENT), Nanjing University, Nanjing 210023, PR China |
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| Abstract: | - 1.1. The time-course of cumene hydroperoxide-induced changes in lipid peroxidation, protein sulfhydryl groups and chemiluminescence intensity was determined in human erythrocytes.
- 2.2. Increase in lipid peroxidation was maximal within 60 min of incubation and was paralleled by a decrease in protein sulfhydryl groups and an increase in chemiluminescence formation.
- 3.3. A standard assay system was established to investigate the protective effects of antioxidants and scavenger compounds on cumene hydroperoxide-induced chemiluminescence formation.
- 4.4. Chain-breaking antioxidants (i.e. butylated hydroxytoluene) and sulfhydryl compounds (i.e. dithiothreitol) were able to suppress chemiluminescence formation.
- 5.5. Our results suggested that secondary free radicals, as well as sulfhydryl groups of proteins are involved in cumene hydroperoxide-induced chemiluminescence formation.
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