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The importance of the surface charge on support media for microbial adhesion
Affiliation:1. Department of Applied Chemistry, Faculty of Engineering, Kumamoto University, 2-39-1 Kurokami, Kumamoto-City, Kumamoto 860, Japan;2. Nittetsu Mining Co. Ltd., New Material Department of Research and Development Center, 8-10-16, Shimorenjaku, Mitaka-City, Tokyo 181, Japan;1. Interdisciplinary Graduate School, Nanyang Technological University, Singapore;2. School of Chemical and Biomedical Engineering, Nanyang Technological University, 637459, Singapore;3. Singapore Membrane Technology Center, Nanyang Environment and Water Research Institute, Nanyang Technological University, 637141, Singapore;4. School of Civil and Environmental Engineering, Nanyang Technological University, 639798, Singapore;5. Advanced Environmental Biotechnology Center, Nanyang Environment and Water Research Institute, Nanyang Technological University, 637141, Singapore;1. Université Clermont Auvergne, Institut Pascal, BP 10448, F-63000 Clermont-Ferrand, France;2. CNRS, UMR 6602, IP, F-63178 Aubière, France;1. Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China;2. School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, China
Abstract:Using cristobalites treated at various temperatures, methane fermentation tests were carried out in an anaerobic fluidized-bed reactor (AFBR). Cristobalites treated below 1,000°C had almost the same qualities in terms of components, physical properties, and surface structures. However, points of zero charge were different between cristobalites treated below 600°C and those treated above 800°C. Points of zero charge on the former numbered about 5 and their surfaces were positively charged in the fermentor controlled pH at 7, while points of zero charge of the latter numbered above 8 and surfaces were negatively charged. In the methane fermentation tests, a TOC removal efficiency of 78% was obtained using positively charged cristobalite at a TOC loading rate of 8 g/l-h, but with negatively charged cristobalite, the efficiency was only 63% under the same conditions. This result confirms the importance of the surface charge on the support medium for the anaerobic digestion in an AFBR.
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