New immobilization method of mammalian cells using alginate and polyacrylate |
| |
Institution: | 1. Department of Transplant Medicine, National Cerebral and Cardiovascular Center;2. Department of Radiology, National Cerebral and Cardiovascular Center;3. Department of Cardiac Surgery, National Cerebral and Cardiovascular Center, Suita, Osaka, Japan |
| |
Abstract: | Mouse-mouse hybridoma cells were immobilized in polyacrylate-alginate gels. The immobilized hybridoma cells were cultured semi-continuously using a fluidized bed reactor, and allowed continuous antibody production without any gel destruction for one month. It has been proved that the polyacrylate-alginate gels were tolerant against physical stress. The composition of the gels suitable for cell growth and antibody production was given as follows; viscosity of alginate at 1% solution: 60–100 cP, alginate concentration: 0.8%, and polyacrylate concentration: 0.2%. In the semi-continuous culture using gels prepared under suitable conditions, the viable cell number was estimated as 2.5×107 cells/ml-gel, and the antibody production rate was 2.2 mg/ml-gel/d, at maximum. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|