High-efficiency transformation of Bacillus subtilis NB22, an antifungal antibiotic iturin producer,by electroporation |
| |
| Affiliation: | 1. Graduate Program in Biosciences, Life Science Division, University of Guanajuato Campus Irapuato-Salamanca. Irapuato, Guanajuato 36500, Mexico;2. Food Department, Life Science División, University of Guanajuato Campus Irapuato-Salamanca, Irapuato, Guanajuato 36500, Mexico;3. Department of Chemistry, Division of Natural and Exact Sciences, University of Guanajuato Campus Guanajuato, 36250, Mexico;4. Department of Biological Sciences, California Baptist University, 8432 Magnolia Avenue, Riverside, CA 92504, USA;5. Department of Entomology, University of California, Riverside, CA 92521, USA |
| |
| Abstract: | High-voltage electroporation was used to transform Bacillus subtilis NB22, an antifungal antibiotic producer, reaching the efficiency of 107 transformants/μg plasmid DNA. Transformation frequency was dependent on the composition of the electroporation solution, the electrical field strength and the cell concentration. Addition of polyethylene glycol (PEG) and mannitol in the transformation solution was critical for a high efficiency of transformation. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
