The stimulatory effect of catalase on the formation in vitro of ethylene by an ethylene-forming enzyme purified from Penicillium digitatum |
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| Affiliation: | 1. Department of Applied Microbial Technology, The Kumamoto Institute of Technology, Ikeda 4-22-1, Kumamoto-shi, Kumamoto 860, Japan;2. Department of Industrial Chemistry, The Kumamoto Institute of Technology, Ikeda 4-22-1, Kumamoto-shi, Kumamoto 860, Japan;1. Laboratory of Reproductive and Developmental Biology, Graduate School of Life Science, Hokkaido University, Sapporo, 060-0810 Japan;2. Laboratory of Reproductive and Developmental Biology, Department of Biological Sciences, Faculty of Science, Hokkaido University, Sapporo 060-0810, Japan;1. Enteric NeuroScience Program, Mayo Clinic School of Medicine, Rochester, Minnesota;2. Department of Integrative Physiology and Pharmacology, Liberty University College of Osteopathic Medicine, Lynchburg, Virginia;1. Department of Bioproducts & Biosystems Engineering, University of Minnesota, Saint Paul, MN 55108, USA;2. Institute on the Environment, University of Minnesota, Saint Paul, MN 55108, USA;3. Department of Ecology, Evolution, and Behavior, University of Minnesota, Saint Paul, MN 55108, USA;4. Department of Plant Biology, University of Minnesota, Saint Paul, MN 55108, USA;1. Department of Mechanical and Aerospace Engineering – Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino, Italy;2. Structural Mechanics and Concepts Branch – NASA Langley Research Center, Mail Stop 190, Hampton, VA 23681-2199, USA;1. Carrera de Ingeniería Forestal, Laboratorio de Dendrocronología, Facultad de Ciencias Agrícolas, Universidad Autónoma Gabriel René Moreno, Km 6 carretera al Norte, El Vallecito, Santa Cruz, Bolivia;2. Departamento de Dendrocronología e Historia Ambiental, Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales (IANIGLA), CCT-CONICET-Mendoza, CC.330 (5500) Mendoza, Argentina;3. Research School of Biology, The Australian National University, Building 46, Acton, ACT 0200, Australia;4. Dpto. Ingeniería Forestal, Laboratorio de Dendrocronología, Universidad de Córdoba, Campus de Rabanales, Ctra. N-IV, Km 396, 14,071 Córdoba, Spain |
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| Abstract: | The dependence of the rate of formation of ethylene on the concentration of an ethylene-forming enzyme was determined with a purified preparation of the ethylene-forming enzyme from Penicillium digitatum IFO 9372. The relationship was n ot linear. When catalase and bovine serum albumin were added to the reaction mixture, the rate of formation of ethylene was directly proportional to the concentration of the enzyme. The non-linearity of the reaction, in the absence of these additives, is probably due to the hydroxyl radical ions (OH ) produced by the Fenton reaction which occurs in the reaction mixture when ferrous ions and oxygen are present together under reducing conditions. |
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