Susceptibility testing of anaerobic bacteria: A review of current methods and future prospects |
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| Institution: | 1. International Health Management Associates, Inc., Schaumburg, IL, USA;2. Drug Discovery & Disease Research Laboratory, Shionogi & Co., Ltd., Osaka, Japan;3. Pharmaceutical Research Division, Shionogi & Co., Ltd., Osaka, Japan;4. Clinical Development and Medical Affairs, ID3C, LLC, Easton, CT, USA;5. Department of Medical Microbiology and Infectious Diseases, College of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada;1. El Colegio de la Frontera Sur (ECOSUR), C. P. 30700, Tapachula, Chiapas, Mexico;2. Centro Regional de Investigación en Salud Pública, Instituto Nacional de Salud Pública (CRISP-INSP), C. P. 30700, Tapachula, Chiapas, Mexico;3. Distrito de Salud VII, Coordinación de Vectores, Servicios Estatales de Salud Chiapas, C. P. 30700, Tapachula, Chiapas, Mexico;4. Consejo Nacional de Ciencia y Tecnologí?a (Investigadora por México) comisionada en El Colegio de la Frontera Sur, C. P. 30700, Tapachula, Chiapas, Mexico |
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| Abstract: | The current NCCLS document, M11 A2, describes two methods for susceptibility testing of anaerobic bacteria. The reference method utilizes an agar dilution procedure, which is labor intensive and not convenient for testing individual patient isolates. The broth microdilution method does not support the growth of 15–40% clinical isolates and demonstrates poor correlation with the reference method for some members of the Bacteroides fragilis group with β-lactam agents and clindamycin. Etest is a new technique that incorporates an antibiotic gradient onto a plastic strip and utilizes agar media. This method is easily performed, permits growth of all anaerobes, and provides quantitative MICs for rapidly growing strains after overnight (20 hr) incubation. This method is convenient and reliable and enables the laboratory to provide the clinician with MIC data for individual patient isolates within a clinically relevant time period. |
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