The effect of cryopreservation and various cryodiluents on allozyme variation of glucose phosphate isomerase in the F1 progency of african catfish (clarias gariepinus) |
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| Institution: | 1. Department of Physiology and Ecotoxicology, Faculty of Biotechnology and Environmental Sciences, The John Paul II Catholic University of Lublin, Kontstantynow 1 “I”, 20-708 Lublin, Poland;2. Department of Biochemistry Environmental Chemistry, Faculty of Biotechnology and Environmental Sciences, The John Paul II Catholic University of Lublin, Kontstantynow 1 “I”, 20-708 Lublin, Poland;1. College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China;2. Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing 210095, China;1. Núcleo de Investigación en Producción Alimentaria, Departamento de Ciencias Biológicas y Químicas, Facultad de Recursos Naturales, Universidad Católica de Temuco, Temuco, Chile;2. Laboratorio de Biotecnología, Instituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile, Santiago, Chile;3. Departamento de Ingeniería Química, Facultad de Ingeniería y Ciencias, Universidad de La Frontera, Temuco, Chile;4. Núcleo de Investigación en Producción Alimentaria, Departamento de Ciencias Agropecuarias y Acuícolas, Facultad de Recursos Naturales, Universidad Católica de Temuco, Temuco, Chile;5. Biochemical and Pharmaceutical Technology Department, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil;6. Programa de Fisiopatología, Instituto de Ciencias Biomédicas (ICBM), Facultad de Medicina, Universidad de Chile, Av. Salvador 486, Providencia, Santiago 7500922, Chile;7. Departamento de Medicina Interna Oriente, Facultad de Medicina, Universidad de Chile, Santiago, Chile;8. Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, Brazil |
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| Abstract: | - 1.1. African sharptooth catfish individuals, heterozygous for glucose phosphate isomerase (GPI-2), were selected as broodstock by using horizontal starch-gel electrophoresis.
- 2.2. Ova of one heterozygous female were inseminated with cryopreserved and fresh milt from a corresponding male.
- 3.3. Significant deviations from expected Hardy-Weinberg proportions occured for offspring obtained by using cryopreserved milt.
- 4.4. Differences in genotypic variation seems to relate to different cryodiluents and the fertility thereof.
- 5.5. Selection of breeding stock for aquacultural practices based on the above information is discussed.
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